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Part:BBa_Y1001:Design

Designed by: Tom Knight   Group: Part Editors   (2006-08-03)


Yeast autonomous replicating sequence (ARS1)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 209
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 423
    Illegal PstI site found at 209
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 238
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 209
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 209
    Illegal NgoMIV site found at 453
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

must eliminate biobrick and PvuII sequences for use in transposome construction.


Source

yeast chromosome 4 (NC_001136, bases 462351-463189), pYAC4

References